293 N3S

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human kidney
• embalagem: tube of 5 μg 92052131-DNA-5UG; pkg of vial of cells 92052131-1VL
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Not specified
• produtos: Adenovirus 5 early region (E1) antigens
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human embryo kidney

Descrição de linhagem celular

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Meio de cultura

Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.

Rotina de subcultura

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO₂; 37°C. Cell doubling time is 30 hours.

Outras notas

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