PI.1 Ut

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: raccoon uterus
• embalagem: tube of 5 μg 90111909-DNA-5UG; pkg of vial of cells 90111909-1VL
• modo de crescimento: Adherent
• cariótipo: 2n = 38
• morfologia: Fibroblast-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Racoon uterus

Descrição de linhagem celular

Derived from trypsinised uterine tissue of an adult female Racoon. Pl.1Ut was developed for studies of feline and canine viruses since the Racoon is one of the few animals which is susceptible to both.

Aplicação

Virus studies: feline and canine viruses

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² by washing 3 times with 0.255 trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; maximum 90 passages expected. Growth rate is very slow.

Outras notas

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