DEM human cell line

IHW Number: 9007, 88052007

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human blood
• etnia: German
• sexo: male
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice

Descrição

Descrição geral

Consanguineous: No

Homozygous: No

Descrição de linhagem celular

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Perfil do DNA

A: 201
Cw: 602
B: 5701
HLA DRA: 101
HLA DRB1: 0401 1602
HLA DRB4: 101
HLA DRB5: 2
HLA DRB6: 202
HLA DQA1: 0102 03
HLA DQB1: 0502 0302
HLA DPA1: 1
HLA DPB1: 0301 0401

Perfil sorológico

HLA A: 2
HLA A ief: 2.2
HLA C: 6
HLA B: 57
HLA B ief: 57.2
Bw4/bw6: 4
HLA DR: 4 16
HLA DR51: 51
HLA DR53: 53
HLA DQ: 5 8
HLA DP: 3 4

Meio de cultura

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rotina de subcultura

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Exoneração de responsabilidade

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.