Jurkat E6.1 Cell Line human

88042803, human blood (leukemic T-cell lymphoblast), Not specified

• Sinônimo(s): Jurkat Cells, Jurkat Clone E6-1 Cells
• Código UNSPSC: 41106514

Propriedades

• Nome do produto: Jurkat E6.1 Cell Line human, 88042803, from human blood(leukemic T-cell lymphoblast)
• fonte biológica: human blood (leukemic T-cell lymphoblast)
• modo de crescimento: Suspension
• cariótipo: Pseudodiploid, modal No 46
• morfologia: Not specified
• produtos: Interleukin 2 (IL-2), human alpha interferon
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human leukaemic T cell lymphoblast

Descrição de linhagem celular

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

Aplicação

IL-2 assay and cloning studies

Jurkat E6.1 cell line has been used to:

• study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
• determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
• study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

Outras notas

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