Detroit 562

87042205, human pharynx, Heteroploid

• Código UNSPSC: 41106514

Propriedades

• product name: Detroit 562, 87042205
• fonte biológica: human pharynx
• modo de crescimento: Adherent
• cariótipo: 2n = 46
• morfologia: Heteroploid
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian pharynx carcinoma

Descrição de linhagem celular

Derived from pleural fluid of an adult female with primary carcinoma of the pharynx. The cells have the type B glucose-6-phosphate dehydrogenase.

Aplicação

Tumourigenicity studies

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
THO1: 8,9
TPOX: 8,10
vWA: 16

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Outras notas

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