Detroit 532

87032602, human skin, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• product name: Detroit 532, 87032602
• fonte biológica: human skin
• modo de crescimento: Adherent
• cariótipo: 2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian, skin, Down syndrome

Descrição de linhagem celular

Derived from the foreskin of a 2 month old Caucasian with Down Syndrome.

Aplicação

Virus studies

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, up to 30 passages expected.

Outras notas

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