EIII

86072804, Human x Mouse Hybridoma hybrid, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: EIII, 86072804, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• fonte biológica: Human x Mouse Hybridoma hybrid
• embalagem: tube of 5 μg 86072804-DNA-5UG; pkg of vial of cells 86072804-1VL
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Fibroblast
• produtos: Not specified
• receptores: pJ3.11, p79a, CFTR DNA markers
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human x mouse hybrid

Descrição de linhagem celular

This cell line may contain a sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.

Aplicação

Somatic cell hybrid mapping

Meio de cultura

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Outras notas

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