BW5147.G.1.4.OUA/R.1

85082302, mouse blood, Lymphoblast

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: BW5147.G.1.4.OUA/R.1, 85082302
• fonte biológica: mouse blood
• embalagem: tube of 5 μg 85082302-DNA-5UG; pkg of vial of cells 85082302-1VL
• modo de crescimento: Suspension
• morfologia: Lymphoblast
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

For complete product information, please see BW5147.G.1.4.OUA/R.1

Origem de linhagem celular

Mouse AKR/J T cell lymphoma

Descrição de linhagem celular

This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.

Aplicação

Fusion partner

Meio de cultura

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 1-6x100,000 cells/ml; 5% CO₂; 37°C.

Outras notas

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