RAJI′A′

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85022102, human lymph, Fibroblast-like

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human lymph
• embalagem: tube of 5 μg 85022102-DNA-5UG; pkg of vial of cells 85022102-1VL
• modo de crescimento: Suspension
• cariótipo: Modal no. 47-48
• morfologia: Fibroblast-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Burkitt′s lymphoma

Descrição de linhagem celular

Clone of RAJI selected for adherence to glass or plastic. Fibroblast like cell line. RAJI ′A′ was developed from the adherent sub-population of RAJI cells. Karyotype analysis demonstrated that RAJI ′A′ contains typical human chromosomes and the modal total chromosome number (47-48) is the same as that of RAJI. RAJI ′A′ is also identical to RAJI with respect to isoenzyme composition, susceptibility to EBV infection and inducibility of latent EBV. Ethnicity: Black.

Aplicação

Tumourigenicity studies

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 17,19

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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