BHK21C13-3P

84100501, hamster kidney, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• product name: BHK21C13-3P, 84100501
• fonte biológica: hamster kidney
• modo de crescimento: Adherent
• cariótipo: 2n = 44, pseudodiploid
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Hamster Syrian kidney

Descrição de linhagem celular

BHK21C13-3P is a subclone of the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells grow as monolayers and have the same virus susceptibility as the parent line.

Aplicação

Virus replication studies

Meio de cultura

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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