OX-97

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: Mouse x Rat Hybridoma
• embalagem: tube of 5 μg 13082218-DNA-5UG; pkg of vial of cells 13082218-1VL
• ImmunoDon: Rat PVG spleen
• Mieloma: NS1
• Reatividade: Mouse CD22
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição de linhagem celular

The hybridoma cell line OX-97 secretes monoclonal IgG1 antibodies reactive with mouse CD22 domain 2.

Imunogênio

Recombinant CD22

Aplicação

The isotype of the monoclonal antibody produced by this cell line is rat IgG1.

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Rotina de subcultura

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.