OX-46

13082205, from Mouse x Mouse Hybridoma

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Mieloma: NS0/1
• Reatividade: Rat CD48
• técnica(s): cell culture | mammalian: suitable
• Isotipo: IgG2a
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição de linhagem celular

The hybridoma cell line OX-46 secretes monoclonal IgG1 (kappa) antibodies reactive with rat CD48.

Imunogênio

Activated rat T cells

Aplicação

Detection of rat CD48 (cross blocks with OX-45).

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Rotina de subcultura

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.