Rat hybridoma producing antibody against mouse CD45 pan isoform reactive. Cambridge Enterprise Reference number AB62:www.enterprise.cam.ac.uk.
Imunogênio
CBA mouse bone marrow
Meio de cultura
IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Rotina de subcultura
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Outras notas
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Exoneração de responsabilidade
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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Certificados de análise (COA)
Lot/Batch Number
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