ONE58 cell line human

10092313, human esophagus, Epithelial-like: cells are spindle-shaped with few vacuoles.

• Código UNSPSC: 41106514

Propriedades

• product name: ONE58 cell line human, 10092313
• fonte biológica: human esophagus
• descrição: Human malignant mesothelioma
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial-like: cells are spindle-shaped with few vacuoles.
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human malignant mesothelioma

Descrição de linhagem celular

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. ONE58 is tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0143

Aplicação

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Meio de cultura

RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS

Rotina de subcultura

Optimal split ratio 1:4 (seeding density 1.2 x 104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Outras notas

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia