C9983
Creatine Kinase BB Fraction human
≥90% (SDS-PAGE), liquid, recombinant, expressed in Pichia pastoris
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Descrição geral
Solution in 50% glycerol, Tris buffer with 10 mM Bis-Tris-HCl, 0.5mM DTT, 0.5mM EDTA
Aplicação
Human creatine kinase BB fraction has been used to investigate the survival benefit of the late percutaneous coronary intervention in patients after acute myocardial infarction. Human creatine kinase BB fraction has also been used in a study to analyze protein oxidations and resultant loss of function.
Ações bioquímicas/fisiológicas
The product is used as a control in monitoring levels in cerebrospinal fluid by ELISA. Creatine kinase (CK) BB is a member of the CK gene family and is a cytosolic CK isoform present in the brain. The molecular mass is found to be 35-50 kDa. CKs catalyze the reversible transfer of a phosphoryl group between ATP and creatine. It plays an important role in the regulation of ATP level in neural cells. Therefore, a decrease in CK BB activity is one of the biochemical markers of the CNS cell damage in age-related neurodegenerative diseases such as Alzheimer′s disease, Pick′s disease, and Lewy body dementia.
Definição da unidade
One unit will transfer 1.0 μmole of phosphate from creatine phosphate to ADP per minute at 37 °C (measured at 340 nm as one equimolar amount of NADH produced by coupled reaction).
Aplicação
Nº do produto
Descrição
Preços
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Bozidarka Knezević et al.
Bosnian journal of basic medical sciences, 9(1), 54-58 (2009-03-17)
The impact of late percutaneous coronary intervention (PCI) in the patients after acute myocardial infarction (AMI) on long term mortality remains to be established. At currently, thrombolysis is accepted as standard therapy when PCI is not immediately available. However, PCI
Loïc Dayon et al.
Analytical chemistry, 80(8), 2921-2931 (2008-03-04)
A new 6-plex isobaric mass tagging technology is presented, and proof of principle studies are carried out using standard protein mixtures and human cerebrospinal fluid (CSF) samples. The Tandem Mass Tags (TMT) comprise a set of structurally identical tags which
Paul D Hamilton et al.
Biochemistry and biophysics reports, 22, 100748-100748 (2020-03-11)
Creatine kinase (CK) is an energy storage enzyme that plays an important role in energy metabolism. CK/phosphocreatine functions as an energy buffer and links ATP production sites with ATP utilization sites. Several key mutations in the αA-crystallin (cryaa) and αB-crystallin
Rezwan Siddiquee et al.
Bio-protocol, 11(10), e4019-e4019 (2021-06-22)
Cell-free synthesis is a powerful technique that uses the transcriptional and translational machinery extracted from cells to create proteins without the constraints of living cells. Here, we report a cell-free protein production protocol using Escherichia coli lysate (Figure 1) to
T Talvik et al.
Acta paediatrica (Oslo, Norway : 1992), 84(10), 1183-1187 (1995-10-01)
Creatine kinase isoenzyme BB was determined in cerebrospinal fluid (CSF) in 79 preterm neonates using an original enzyme-linked immunosorbent assay. The criterion for inclusion was an Apgar score of 7 or less at 5 min of life. Neurological examination was
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