C9205

Anticorpo monoclonal anti-proteína ácida fibrilar glial (GFAP)

clone G-A-5, purified from hybridoma cell culture

• Sinônimo(s): Anti-GFAP
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: CY3 conjugate
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: G-A-5, monoclonal
• Formulário: buffered aqueous solution
• reatividade de espécies: human, pig, rat
• técnica(s): direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections
• Isotipo: IgG1
• nº de adesão UniProt: P14136
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... GFAP(2670) ; rat ... Gfap(24387)

Descrição

Descrição geral

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well.

Especificidade

O anticorpo reage especificamente com GFAP em ensaios de immunoblotting e marca astrócitos, células gliais de Bergmann e condrócitos de cartilagem elástica em coloração imuno-histoquímica. O anticorpo reage com o antígeno glial específico em cortes de tecido congelados ou fixados em álcool.

The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Imunogênio

GFAP da medula óssea de suínos

GFAP from pig spinal cord

Aplicação

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in

• immunohistochemistry[1]
• immunoblotting
• immunolabelling,
• immunocytochemistry
• immunofluorescence

Ações bioquímicas/fisiológicas

Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Informações legais

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of Cytiva

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)