C8743
Cholesterol Dehydrogenase from Nocardia sp.
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About This Item
Produtos recomendados
recombinante
expressed in Streptomyces lividans
forma
lyophilized solid
atividade específica
≥18 unit/mg solid
peso molecular
~37 kDa by SDS-PAGE
temperatura de armazenamento
−20°C
Aplicação
The enzyme sequence is derived from Nocardia sp., and this cholesterol dehydrogenase is expressed in Streptomyces lividans. Cholesterol Dehydrogenase from Nocardia sp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase from Nocardia sp. Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains.
Enzyme used in determining cholesterol in serum
Ações bioquímicas/fisiológicas
Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay.
Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5.
Definição da unidade
One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 °C.
forma física
Supplied as a lyophilized powder
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
Certificados de análise (COA)
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Cloning, nucleotide sequence, and transcriptional analysis of the NAD (P)-dependent cholesterol dehydrogenase gene from a Nocardia sp. and its hyperexpression in Streptomyces spp.
Horinouchi S, et al.
Applied and Environmental Microbiology, 57(5) (1991)
Lorant Janosi et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(21), 8097-8102 (2012-05-09)
Recent experiments have shown that membrane-bound Ras proteins form transient, nanoscale signaling platforms that play a crucial role in high-fidelity signal transmission. However, a detailed characterization of these dynamic proteolipid substructures by high-resolution experimental techniques remains elusive. Here we use
Takeshi Nomura et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(22), 8770-8775 (2012-05-16)
Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the major players in the protection of bacterial cells against hypoosmotic shock. Although a great deal is known about structure and function of these channels, much less is known
A disposable amperometric biosensor for determining total cholesterol in whole blood
Fang C, et al.
Sensors and Actuators B, Chemical, 155(2) (2011)
The characteristics and applications of recombinant cholesterol dehydrogenase
Kishi K, et al.
Bioscience, Biotechnology, and Biochemistry, 64(7) (2000)
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