This item is a product of PromoCell. The manufacturer considers the formulation proprietary. However, this is a complete medium that includes all the growth factors and supplements necessary for providing optimal cell health and efficient adherence selection of monocytes from freshly isolated mononuclear cells. This medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.
C-28051
Monocyte Attachment Medium
250 ml
Sinônimo(s):
Primary Cell Culture Media
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.71
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
Formulário
aqueous solution
embalagem
pkg of 250 ml
técnica(s)
cell culture | mammalian: suitable
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Descrição geral
Monocyte Attachment Medium (Ready-to-use)
Aplicação
The Monocyte Attachment Medium allows for the efficient adherence selection of monocytes from freshly isolated human mononuclear cells while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes is dispensable prior to experiments requiring adherent monocytes as a starting material. With properly performed washing steps large numbers of 80-90% pure monocytes can be obtained. Typical applications are generation of dendritic cells / macrophages as well as phagocytosis assays.Recommended for:Human mononuclear cells (freshly isolated)Human monocytes (freshly isolated)
Qualidade
All lots of PromoCell Monocyte Attachment Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from peripheral blood. Each lot of Monocyte Attachment Medium is checked for the efficient adherence selection of highly viable monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Nota de preparo
The Medium is chemically defined/xeno-free and does not need further supplementation.
Armazenamento e estabilidade
Store the Medium at 2 to 8°C in the dark immediately after arrival. Do not freeze the Medium. If stored properly, the product is stable until the expiry date stated on the label. After opening, the shelf life of the medium is 6 weeks at 2 to 8°C. For use, pre-warm only an aliquot of the medium and keep the remaining medium refrigerated at 2 to 8°C.
Rotina de subcultura
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Protocolos
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