Skeletal Muscle Cell Growth Medium is a low-serum medium (5% V/V) developed for the expansion of human skeletal muscle cell cultures. The media are optimized for primary human cells, but can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Recommended for:Human Skeletal Muscle Cells (SkMC)
Componentes
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials
Qualidade
All lots of PromoCell Skeletal Muscle Cell Growth Medium are subjected to comprehensive quality control tests using human primary SkMC. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested SkMC. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Nota de preparo
Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlFetuin (bovine): 50 µg / mlEpidermal Growth Factor (recombinant human): 10 ng / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / mlInsulin (recombinant human): 10 µg / mlDexamethasone: 0.4 µg / ml
Armazenamento e estabilidade
Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Primary human skeletal muscle cells and culture medium optimized for the in vitro cultivation and differentiation of primary human skeletal muscle cells. Protocols for handling, cell passaging, media, and product use.
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