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Documentos Principais

C-12710

Sigma-Aldrich

Human Chondrocytes (HCH)

500,000 cryopreserved cells

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.81
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

human articular cartilage (knee and hip joints)

embalagem

pkg of 500,000 cells

morfologia

(chondrocytes)

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−196°C

Descrição geral

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origem de linhagem celular

Cartilage

Aplicação

Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request) and are provided in a cryopreserved format. HCH are routinely tested for their capacity to differentiate in 3D spheroids.The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).

Qualidade

Rigid quality control tests are performed for each lot of Human Chondrocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, each lot is routinely tested for the capacity to differentiate in 3D spheroids. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV and HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertência

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rotina de subcultura

Click here for more information.

Outras notas

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: Differentiation testedGuaranteed population doublings: >10

Produtos recomendados

Recommended Primary Cell Culture Media:Link

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Artigos

Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.

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