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A8273

Sigma-Aldrich

Anti-α-Amylase antibody produced in rabbit

Anti-α-Amylase antibody produced in rabbit

fractionated antiserum, lyophilized powder

Sinônimo(s):

Alpha Amylase Antibody, Alpha Amylase Antibody - Anti-α-Amylase antibody produced in rabbit

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1 VIAL
R$ 2.554,00

R$ 2.554,00


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1 VIAL
R$ 2.554,00

About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

R$ 2.554,00


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fonte biológica

rabbit

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

fractionated antiserum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

lyophilized powder

reatividade de espécies

human

embalagem

vial of 2 mL lyophilized antiserum

técnica(s)

Ouchterlony double diffusion: suitable
indirect ELISA: 1:4,000-1:6,000

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... AMY1A(276)

Descrição geral

α-amylase is primarily produced in abundance in the salivary glands and pancreas. The expression is also seen in jejunum and mammary glands. The genes encoding α-amylase are AMY1 and AMY2 located in the short arm of chromosome 1. AMY1 is responsible for producing the enzyme in saliva and mammary gland and AMY2 produces the enzyme synthesized in the pancreas. The expression of AMY1 occurs also in certain tumour tissues. Mammalian amylases are composed of three structural domains (A, B and C). Domain A contains the active site with two aspartate and one glutamate residue. Domain B borders the active site region and is essential for maintaining the protein conformation. Domain C is involved in catalytic mechanism.

Especificidade

The antiserum is specific for human a-amylase found in human saliva and human pancreatic extract. No reaction with other human saliva proteins or pancreatic extract proteins is observed.

Imunogênio

human salivary α-amylase.

Aplicação

Anti-α-Amylase antibody produced in rabbit has also been used in immunocytochemistry analysis.
Anti-a-Amylase antibody produced in rabbit has been used in immunofluorescence and immunohistochemical analysis.
Mouse pancreatic sections were fixed in 4% paraformaldehyde and used for immunohistochemistry using rabbit anti-amylase antibody at a dilution of 1:100.

Ações bioquímicas/fisiológicas

α-Amylase is essential for catalyzing the primary step in starch digestion, a main source of carbohydrate in the human diet. α-amylase hydrolysis the polysaccharide with the ultimate production of maltose, maltotriose and limit dextrins as the main products.

forma física

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2

Reconstituição

Reconstitute with 2 mL deionized water.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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  1. 愛知
    • Review 1
    • Vote 1
    4 out of 5 stars.

    マウス唾液アミラーゼにも反応する様です。

    データシートにもマウス唾液アミラーゼにも反応する旨記載頂きたいです。WBで55kDa付近にシングルバンドで検出されます。

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