A6907

Anti-IgM humana (específica de cadeia μ)−Peroxidase

affinity isolated antibody, buffered aqueous solution

• Sinônimo(s): Goat Anti-Human IgM (μ-chain specific)−HRP
• Número MDL: MFCD00162464
• Código UNSPSC: 12352203
• NACRES: NA.46

Propriedades

• fonte biológica: goat
• conjugado: peroxidase conjugate
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: secondary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• reatividade de espécies: human
• concentração: 0.5-4 mg/mL
• técnica(s): direct ELISA: 1:10,000
• Isotipo: IgM
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Human IgM antibodies are glycoproteins that are mainly produced by the B-1 cells and regulate the physiology and growth of the human B cell reserve. Circulating monoclonal IgM antibody that binds to myelin-associated glycoprotein has been linked to the demyelination of peripheral nerves in humans . Anti-human IgM μ-chain specific−peroxidase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones κ and Lambda myeloma proteins.

There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five to six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.

Imunogênio

human IgM

Aplicação

Anti-Human IgM (μ-chain specific)-Peroxidase antibody has been used in direct binding assays to detect antiphospholipid antibodies (aPL).

Anti-human IgM (μ-chain specific)-Peroxidase antibody is suitable for use in direct ELISA (1:10,000 and 1:5000). It has also been used in indirect ELISA (1:2000). The concentration of IgM in supernatant samples was determined by ELISA using HRP-conjugated goat anti-human chain specific IgG as the secondary antibody. The antibody has also been used in Western blotting.

Ações bioquímicas/fisiológicas

Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% BSA with preservative.

Nota de preparo

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS07,GHS09
• Palavra indicadora: Warning
• Frases de perigo: H315,H317,H319,H411
• Declarações de precaução: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable