A5268

Alcian Blue 8GX

powder

• Sinônimo(s): Alcian Blue, Ingrain Blue 1
• Fórmula empírica (Notação de Hill): C₅₆H₆₈Cl₄CuN₁₆S₄
• Número CAS: 33864-99-2
• Peso molecular: 1298.86
• Número do índice de cores: 74240
• Número CE: 251-705-7
• Número MDL: MFCD00010720
• Código UNSPSC: 12171500
• ID de substância PubChem: 24890962
• NACRES: NA.47

Propriedades

• Formulário: powder
• Nível de qualidade: 100
• composição: Dye content, 45-65%
• técnica(s): microbe id | staining: suitable
• cor: blue
• solubilidade: H₂O: 1 mg/mL
• εmax: 425 at 615-630 nm in water
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: [Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C
• InChI: 1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4
• chave InChI: CKLBXIYTBHXJEH-UHFFFAOYSA-J

Descrição

Descrição geral

Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.[1]

Aplicação

• Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.[2][3]
• It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models.[4]
• It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species.[5]

Características e benefícios

Desired staining patterns can be obtained by manipulating pH and salt concentrations.[1]

Princípio

The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)