A0185
Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse
clone BN-34, purified immunoglobulin
Sinônimo(s):
Monoclonal Anti-Biotin
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1 VIAL
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1 VIAL
R$ 3.013,00
About This Item
Código UNSPSC:
12352203
NACRES:
NA.46
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
peroxidase conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
BN-34, monoclonal
embalagem
vial of 0.5 mL
técnica(s)
dot blot: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
indirect ELISA: 1:30,000
western blot (chemiluminescent): 1:80,000
Isotipo
IgG1
temperatura de armazenamento
2-8°C
modificação pós-traducional do alvo
unmodified
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Descrição geral
Monoclonal Anti-Biotin (mouse IgG1 isotype) has been derived from the BN-34 hybridoma produced by the used successfully for detection of micro-injected, fusion of mouse myeloma cells and splenocytes from biotin-haptenized cytoskeletal proteins which enable BALB/c mice immunized with biotinylated keyhole limpet hemocyanin (KLH).
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Imunogênio
Biotin-KLH conjugate
Aplicação
May be substituted for avidin to decrease non-specific binding.
Monoclonal Anti-Biotin Peroxidase antibody produced in mouse has been used:
- in immunohistochemistry
- in western blot
- as secondary antibody to detect the presence of aptamer on the membrane
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Ações bioquímicas/fisiológicas
Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
Monoclonal Anti-Biotin-Peroxidase antibody specific for biotin conjugated to various proteins. The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. The product specificity has been evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Monoclonal Anti-Biotin-Peroxidase antibody specific for biotin conjugated to various proteins. The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. The product specificity has been evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
forma física
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Sens. 1
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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