84120

Safranin O

for microscopy (Bact., Bot., Hist.), indicator (pH 0.3-1.0)

• Sinônimo(s): Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
• Fórmula empírica (Notação de Hill): C₂₀H₁₉ClN₄
• Número CAS: 477-73-6
• Peso molecular: 350.84
• Número do índice de cores: 50240
• Beilstein: 3924099
• Número CE: 207-518-8
• Número MDL: MFCD00011759
• Código UNSPSC: 12171500
• ID de substância PubChem: 329769290
• NACRES: NA.47

Propriedades

• grau: for microscopy (Bact., Bot., Hist.); indicator (pH 0.3-1.0)
• forma: powder or crystals
• técnica(s): titration: suitable
• pH: 10 (20 °C, 10 g/L)
• solubilidade: methanol: 0.01 g/10 mL, red
• εmax: ≥1000 at 525-535 nm in 50% ethanol
• aplicação(ões): diagnostic assay manufacturing; hematology; histology; microbiology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
• chave InChI: OARRHUQTFTUEOS-UHFFFAOYSA-N

Descrição

Descrição geral

Safranin O, also known as Basic Red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalized positive charge, an alternation of aromatic and quinonoid structures, and diazotizable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.

Aplicação

• Safranin O is widely employed as a microbiological stain, especially as a counterstain in Gram staining to distinguish Gram-positive from Gram-negative micro-organisms, and for staining bacterial spores.
• It is used to demonstrate glycosaminoglycans in human and animal histological specimens and nuclei.
• It is a component of Benda′s polychrome stain.
• It is a component of various polychromes and lignin stains for botanical staining applications.
• It has also been applied for the demonstration of mast cells in cytospin preparations and staining surgical frozen sections.

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Danger
• Frases de perigo: H318
• Declarações de precaução: P280 - P305 + P351 + P338
• Classificações de perigo: Eye Dam. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves