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Sigma-Aldrich

4-Methylumbelliferyl-β-D-glucuronide hydrate

β-glucuronidase substrate, fluorogenic, ≥99.0% (HPLC), powder or crystals, suitable for fluorescence

Sinônimo(s):

4-Methylumbelliferyl-β-D-glucopyranosiduronic acid, MUG

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About This Item

Fórmula empírica (Notação de Hill):
C16H16O9 · xH2O
Número CAS:
881005-91-0
Peso molecular:
352.29 (anhydrous basis)
Número CE:
228-186-0
Número MDL:
MFCD09039280
Código UNSPSC:
12352204
ID de substância PubChem:
57652201
NACRES:
NA.32

product name

4-Methylumbelliferyl-β-D-glucuronide hydrate, suitable for fluorescence, ≥99.0% (HPLC)

Ensaio

≥99.0% (HPLC)

forma

powder or crystals

Impurezas

≤0.1% free 4-Methylumbelliferone
≤15% water

solubilidade

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

fluorescência

λex 365 nm; λem 445 nm in 0.1 M phosphate pH 6.5

adequação

suitable for fluorescence

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

O.CC1=CC(=O)Oc2cc(O[C@@H]3O[C@@H]([C@@H](O)[C@H](O)[C@H]3O)C(O)=O)ccc12

InChI

1S/C16H16O9.H2O/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22;/h2-5,11-14,16,18-20H,1H3,(H,21,22);1H2/t11-,12-,13+,14-,16+;/m0./s1

chave InChI

URVSQZMOFUEQAW-YYHOVTOASA-N

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Descrição geral

4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) is a fluorogenic substrate.

Aplicação

4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) has been used:
  • as a β-glucuronidase (GUS) substrate for fluorimetric analysis of reporter gene expression in Arabidopsis thaliana
  • for the fluorimetric assay for GUS activity
  • to measure liposome lysis by n-octylglucoside

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Nathaniel M Butler et al.
Planta, 236(6), 1747-1755 (2012-08-08)
Polypyrimidine tract-binding (PTB) proteins are RNA-binding proteins that target specific RNAs for post-transcriptional processing by binding cytosine/uracil motifs. PTBs have established functions in a range of RNA processes including splicing, translation, stability and long-distance transport. Six PTB-like genes identified in
R.B. Thompson et al.
Analytical Letters, 18, 1847-1847 (1985)
I George et al.
Canadian journal of microbiology, 47(7), 670-675 (2001-09-08)
Two enzymatic methods based on the measurement of the beta-D-galactosidase activity of total coliforms and the beta-D-glucuronidase activity of Escherichia coli were used to assess coliform levels in wastewater alongside traditional culture-based techniques, which can be biased by the aggregation
Zeng Tao et al.
Nature plants, 5(4), 424-435 (2019-04-10)
Some overwintering plants acquire competence to flower, after experiencing prolonged cold in winter, through a process termed vernalization. In the crucifer plant Arabidopsis thaliana, prolonged cold induces chromatin-mediated silencing of the potent floral repressor FLOWERING LOCUS C (FLC) by Polycomb
Jean-Félix Dallery et al.
Journal of experimental botany, 71(10), 2910-2921 (2020-02-02)
Infection of Arabidopsis thaliana by the ascomycete fungus Colletotrichum higginsianum is characterized by an early symptomless biotrophic phase followed by a destructive necrotrophic phase. The fungal genome contains 77 secondary metabolism-related biosynthetic gene clusters, whose expression during the infection process

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