This product is pooled from a bovine source. The bile salt mixture is composed of approximately 50% each of sodium deoxycholate and sodium cholate. The exact composition is not tested. The manufacturing process is consistent, however, given the nature of the source, some variability between lots is expected. The degree of variability does not negatively impact microbial growth. Please see section 3 of the Safety Data Sheet:
https://www.sigmaaldrich.com/sds/sial/b3426
B3426
Bile salts mixture
suitable for microbiology
Sinônimo(s):
Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture
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About This Item
Código UNSPSC:
41106212
NACRES:
NA.74
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
esterilidade
non-sterile
Nível de qualidade
Formulário
powder
embalagem
pkg of 100 g
perda
≤6% loss on drying
cor
yellow to brownish-yellow
pH
7±0.5
solubilidade
distilled water: freely soluble
aplicação(ões)
microbiology
temperatura de armazenamento
10-30°C
Descrição geral
Bile salts mixture is a combination of an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Bile salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic, and lithocholic acids. Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that inhibits Gram-positive bacteria and induced germination. It also inhibits the outgrowth of the germinated spores but does not interfere with the growth of Enterobacteriaceae.
Aplicação
Bile salts mixture is recommended in diverse media like MacConkey Agar, SS Agar, and Violet Red Bile Agar. It increases the selectivity of the media to Enterobacteriaceae by inhibiting Gram-positive bacteria. It is used as a selective agent in culture media for the selective growth of intestinal bacteria like E. coli, other fecal coliforms, and fecal Streptococci. But it is also used as a selective agent in media for the detection of Yersinia enterocolitica, Aeromonas hydrophila, and Vibrio species.
Nota de preparo
Extracted from purified, fresh bile.
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Chhatra B Kunwar et al.
Microbiology (Reading, England), 166(12), 1149-1159 (2020-11-19)
Colonization of the gastrointestinal tract by enterohaemorrhagic Escherichia coli (EHEC) is critically dependent on its ability to sense and respond to various microenvironments within the host. EHEC exposure to physiologically relevant levels of bile salts upregulates the two-component system, pmrAB
Crystal Gadishaw-Lue et al.
Infection and immunity, 89(2) (2020-11-25)
During passage through the human gastrointestinal tract, enterohemorrhagic Escherichia coli (EHEC) is exposed to membrane-damaging bile in the small intestine. We previously reported that EHEC treatment with a physiological bile salt mixture upregulates basRS, encoding a two-component system, and arnBCADTEF
Artigos
Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.
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What us the bile salt composition of this mixture? Is this constant between batches?
1 answer-
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