85927

ECC ChromoSelect Selective Agar

suitable for microbiology, NutriSelect^® Plus

• Sinônimo(s): E. coli-Coliform Selective Agar ChromoSelect, ECC Selective Agar ChromoSelect
• Número MDL: MFCD01634645
• Código UNSPSC: 41171606
• NACRES: NA.85

Propriedades

• esterilidade: non-sterile
• Nível de qualidade: 200
• forma: powder
• prazo de validade: limited shelf life, expiry date on the label
• composição: agar, 10.0 g/L ; casein enzymic hydrolysate, 3.3 g/L ; chromogenic mixture, 0.43 g/L ; disodium hydrogen phosphate, 1 g/L ; peptone, special, 6.0 g/L ; sodium chloride, 2 g/L ; sodium dihydrogen phosphate, 0.6 g/L ; sodium pyruvate, 1 g/L ; sorbitol, 1 g/L ; Tergitol^™; 7, 0.15 g/L ; tryptophan, 1 g/L
• fabricante/nome comercial: NutriSelect^® Plus
• pH final: 6.8±0.2 (25 °C)
• aplicação(ões): agriculture; bioburden testing; environmental; food and beverages; water monitoring; microbiology
• adequação: selective and differential for Citrobacter spp.; selective and differential for Enterobacter spp.; selective and differential for Escherichia coli; selective and differential for Salmonella spp.; selective and differential for Shigella spp.; selective and differential for coliforms; selective and differential for enterobacteriaceae

Descrição

Aplicação

ECC ChromoSelect Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol^™ inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.

Nota de preparo

Suspend 26.5 g in 1 litre distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species add 5 mg Cefsulodin (Cat. No. 22126) for surface and pour plate method and 10 mg Cefsulodin for membrane filter technique. Mix well.

Reconstituição

Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of cefsulodin for surface and pour plate method and 10 mg of cefsulodin for membrane filter technique. Mix well.

Nota de rodapé

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informações legais

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)