76704

modified Tryptic Soy Broth- Dehydrated Culture Media

NutriSelect^® Plus, novobiocin 0.02 g/L, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC)

• Sinônimo(s): Modified Tryptic Soy Broth with Novobiocin, Modified Tryptic Soy Broth; EHEC enrichment broth, mTSB Broth with Novobiocin
• Código UNSPSC: 41171607
• NACRES: NA.85

Propriedades

• Agency: according to ISO 16654:2001
• Nível de qualidade: 200
• esterilidade: non-sterile
• forma: powder
• prazo de validade: limited, expiry date on the label
• composição: bile salts No. 3, 1.5 g/L ; di-potassium hydrogen phosphate, 4.0 g/L ; D(+)-glucose, 2.5 g/L ; novobiocin, 0.02 g/L ; peptone from casein, 17.0 g/L ; peptone from soymeal, 3.0 g/L ; sodium chloride, 5.0 g/L
• fabricante/nome comercial: NutriSelect^® Plus
• embalagem: pkg of -500 g
• condição de armazenamento: (Dry place, tightly sealed containers)
• técnica(s): microbiological culture: suitable
• cor: faint brown
• pH final: 7.3±0.2 (25 °C)
• aplicação(ões): agriculture; food and beverages; microbiology; pathogen testing
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• adequação: selective for Escherichia coli; selective for coliforms; selective for enterovirulent E. coli (EEC)

Descrição

Descrição geral

mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.

Aplicação

mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products.The medium meets the requirements of the DIN Norm 10167 for the detection of E. coli serotype O157:H7 in foods. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli (EHEC).

Nota de preparo

Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

Nota de rodapé

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informações legais

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)