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UC0E05

Sigma-Aldrich

UCOE® Intermediate Vector Set

Sinônimo(s):

UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element, UCOE05

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About This Item

Código UNSPSC:
41106621
eCl@ss:
34360190
NACRES:
NA.51

Condições de expedição

dry ice

Nível de qualidade

100

Categorias relacionadas

Descrição geral

The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 contains the 3 kb mouse Rps3 UCOE and the 1.5 kb fragment of human HNRPA2B1-CBX3 UCOE.
CET 1310 contains the 8 kb fragment of human HNRPA2B1-CBX3 UCOE.
CET 1316 contains the 4 kb fragment of human HNRPA2B1-CBX3 UCOE.

References
Antoniou, M., et al.. (2003) Transgenes Encompassing Dual Promoter CpG Islands from the Human TBP and HNRPA2B1 Loci are Resistant to Heterochromatin-mediated Silencing. Genomics, 82: 269-279.
Antoniou, M., et al. (2010) A Ubiquitous Chromatin Opening Element (UCOE) Confers Resistance to DNA Methylation-mediated Silencing of Lentiviral Vectors. Mol Ther, 18: 1640-1649.

THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.

Componentes

1) 10ug CET 1019 (3 kb Rps3 and 1.5kb HNRPA2B1-CBX3) Vector (CS221300)

2) 10ug CET 1310 (8 kb HNRPA2B1-CBX3) Vector (CS221301)

3) 10ug CET 1316 (4 kb HNRPA2B1-CBX3) Vector (CS221302)

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações legais

UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

12 - Non Combustible Liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Michael Antoniou et al.
Genomics, 82(3), 269-279 (2003-08-09)
The genetic elements that are responsible for establishing a transcriptionally competent, open chromatin structure at a region of the genome that consists only of ubiquitously expressed, housekeeping genes are currently unknown. We demonstrate for the first time through functional analysis
Fang Zhang et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 18(9), 1640-1649 (2010-07-01)
DNA methylation may restrict the activity of gene transfer vectors due to inadvertent silencing. In P19 embryonic carcinoma cells in vitro, we found that transgene expression regulated by the SFFV LTR and EF1 alpha promoter declined rapidly within 16 days

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