Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.
Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
Recognizes the ~70-75 kDa AFP protein in HepG2 cells.
Imunogênio
A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
Aplicação
ELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Advertência
Toxicity: Regulatory Review (Z)
forma física
In PBS, pH 7.4.
Reconstituição
Following initial thaw, aliquot and freeze (-20°C).
Nota de análise
Negative Control 293T cells
Positive Control HepG2 cells
Outras notas
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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