SCT281

BioTracker^™ Blue Live Cell Cycle Assay Solution

Human

• Sinônimo(s): cell cycle dye, cell cycle indicator, cell cycle probe, live cell probe
• Código UNSPSC: 41106514
• NACRES: NA.47

Propriedades

• product name: BioTracker^™ Blue Live Cell Cycle Assay Solution,
• fonte biológica: human
• Nível de qualidade: 100
• embalagem: vial of 50 tests
• fabricante/nome comercial: Millipore
• técnica(s): cell culture | stem cell: suitable; flow cytometry: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Propidium iodide (PI) is often used for cell cycle analysis by flow cytometry. Compared to this analysis method, the BioTracker^™ Far Red Live Cell Cycle Assay Solution does not require cell fixation or permeabilization, and is permeable in live cells. Use 5 uL of probe solution in 500 uL for each sample. Vial contains enough for 50 tests.IMPORTANT APPLICATION NOTES:*Wash cells thoroughly to remove trypsin. Residual trypsin can confound analysis of flow cytometry populations.*Adjust gates after cells are stained with probe SPECTRAL PROPERTIESExcitation: 405 - 407 nmEmission: 450/50 BPUse violet laser for excitation.

Características e benefícios

Blue fluorescence probe for analysis of the cell cycle by flow cytometry.

Descrição-alvo

The control mechanism of the cell cycle is closely linked to cell proliferation. The cell cycle includes cell division, in which one cell produces two daughter cells. The cell cycle can be assessed by detecting cells stained for DNA using flow cytometry, followed by analysis of the proportion of stained nuclei in cell cycle phase. The cell cycle is classified into two major phases: interphase and the mitotic (M) phase. Interphase is composed of G1 phase (preparation for DNA synthesis which occurs prior to cell division), S phase (DNA synthesis and replication), and G2 phase (chromosome duplication). M phase involves mitosis and cytokinesis. Cell cycle progression is regulated by cyclins and cyclin-dependent kinases. To determine the action of drugs such as anticancer therapeutics, it is essential to analyze the mechanism of cell-cycle checkpoint.

Armazenamento e estabilidade

Store BioTracker^™ Blue Live Cell Cycle Assay Solution at -20 °C, protected from light.

Outras notas

Live cell probe for fluorescent cell cycle analysis. Unlike propidium iodide (PI), this probe is membrane permeable and highly specific for DNA, which makes it possible to analyze the cell cycle by simply adding the reagent to a cell suspension without any fixation, permeabilization, or other sample prep.

Informações legais

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS05,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H318,H411
• Declarações de precaução: P273 - P280 - P305 + P351 + P338 - P391 - P501
• Classificações de perigo: Aquatic Chronic 2 - Eye Dam. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable