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Sigma-Aldrich

3dGRO® Wnt3a Conditioned Media Supplement

Sinônimo(s):

Wnt3a Conditioned Media;Wnt3a CM;Wnt Conditioned Media

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.76

embalagem

1 ea of 50 mL

fabricante/nome comercial

Millipore

técnica(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Descrição de linhagem celular

3D organoid culture systems are increasingly employed as powerful tools for the study of human diseases. Wnt proteins hetero-dimerize Frizzled (Fzd) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin resulting in expression of genes regulating cell development. Wnt3a is considered an essential protein in many organoid culture systems (intestinal, pancreatic, liver, colon etc.) because the proliferation of stem cells is governed by the Wnt signaling pathway. However, due to the high concentration requirement in organoid media, the use of the recombinant Wnt3a becomes prohibitively expensive. Additionally, recombinant Wnt3a proteins are often unstable and lack biological activity. The 3dGRO® Wnt3a Conditioned Media Supplement is a potent and inexpensive alternative to purified recombinant Wnt3a protein for organoid cultures and is optimized for the highest level of Wnt3a expression to ensure consistency and performance.SourceThe 3dGRO® Wnt3a Conditioned Media Supplement is generated from mouse L-M(TK-) cells transfected with a mouse Wnt-3A expression vector. The conditioned medium contains fetal bovine serum.

Aplicação

  • Appearance (color): clear, red liquid
  • Endotoxin: 2 EU/mL
  • Sterility Tested: No Growth/Pass
  • Mycoplasma: Negative
  • Media tested for function in supporting growth of human pancreatic organoids
  • Media tested for biological activity using a Wnt3a luciferase reporter assay in HEK293 STF cells

Características e benefícios

Alternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures.

Armazenamento e estabilidade

Wnt3a Conditioned Medium should be stored at -20°C. When ready to use, thaw the supplement overnight at 2-8°C in the dark. Thawed Wnt3a Conditioned Medium is good for two weeks when stored at 2-8°C.

Informações legais

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Artigos

Human Pancreatic Organoids for Cancer Research Applications

Human pancreatic cancer organoid biobank (PDAC organoids) with various KRAS mutations to aide in 3D cell culture and cancer research applications.

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