The Rat Stress Hormone panel was tested with 8 mouse samples (2 each of OB/OB, DB/DB, Swiss Webster, and CD-1), and the analytes in the samples tested were successfully detected. However, it's important to note that the assay conditions were not fully validated or optimized for mice. Therefore, the assay need to be validated specifically for mouse samples.
RSHMAG-69K
MILLIPLEX® Rat Stress Hormone Magnetic Bead Panel - Endocrine Multiplex Assay
This MILLIPLEX MAP Rat Stress Hormone Magnetic Bead Panel is an Endocrine multiplex assay. The analytes available for this multiplex kit are: ACTH, Corticosterone, Melatonin.
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About This Item
Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
Produtos recomendados
Nível de qualidade
reatividade de espécies
rat
fabricante/nome comercial
Milliplex®
assay range
accuracy: 61%
(Corticosterone)
accuracy: 83%
(ACTH)
sensitivity: 0.9 pg/mL
(ACTH)
sensitivity: 1.52 pg/mL
(Melatonin)
sensitivity: 2914 pg/mL
(Corticosterone)
standard curve range: 0.55-400 pg/mL
(Melatonin)
standard curve range: 1.4-1,000 pg/mL
(ACTH)
standard curve range: 686-500,000 pg/mL
(Corticosterone)
inter-assay cv: <15%
intra-assay cv: <10%
técnica(s)
multiplexing: suitable
método de detecção
fluorometric (Luminex xMAP)
Condições de expedição
wet ice
Descrição geral
Stress is defined as the disruption of homeostasis through physical and/or psychological stimuli orstressors. Physiologically the neuroendocrine system, which includes the hypothalamus, pituitary and adrenal glands, plays a critical role in response to stress. Activated by corticotropin-releasing hormone (CRH) and arginine-vasopressin (AV), the pituitary gland releases ACTH which results in the secretion of cortisol / corticosterone and other glucocorticoids from the adrenal glands. These corticoids involve the whole body in the stress response and are involved in the termination of the stress response by a negative feedback loop to the hypothalamus. Corticosterone and melatonin are important in the regulation of the sleep-wake cycle.
MILLIPLEX® Rat Stress Hormone Panel is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue/cell extracts or culture media samples: ACTH, corticosterone and/or melatonin. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
MILLIPLEX® Rat Stress Hormone Panel is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue/cell extracts or culture media samples: ACTH, corticosterone and/or melatonin. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
Especificidade
Cross Reactivty
This assay display insignificant cross-reactivity within the panel
This assay display insignificant cross-reactivity within the panel
Aplicação
- Analytes: ACTH, Corticosterone, Melatonin
- Recommended Sample Type: Rat serum, plasma, cell/tissue supernatants and lysates
- NOTE: For optimal assay performance of turbid serum and plasma samples an extraction protocol is provided.
- Recommended Sample Dilution: 25μL per well is used for the following: undiluted extracted serum or plasma; 1:4 non-extracted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Endocrine
- Research Subcategory: Metabolism
Características e benefícios
Design your multiplex kit by choosing available analytes within this panel.
Outras notas
Please contact Technical Service for linearity of dilution.
Informações legais
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Código de classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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