RADPCMAG-82K
MILLIPLEX® Rat Adipocyte Panel - Metabolism Assay
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Produtos recomendados
reatividade de espécies
rat
Nível de qualidade
fabricante/nome comercial
Milliplex®
assay range
accuracy: 94-121%
sensitivity: 0.1-25 pg/mL
(MinDC+2SD)
standard curve range: 0.7-3,000 pg/mL
(IL-1β, TNFα)
standard curve range: 12-50,000 pg/mL
(PAI-1)
standard curve range: 122-500,000 pg/mL
(Adiponectin)
standard curve range: 15-60,000 pg/mL
(IL-6, Leptin)
standard curve range: 9.8-40,000 pg/mL
(MCP-1)
técnica(s)
multiplexing: suitable
método de detecção
fluorometric (Luminex xMAP)
Condições de expedição
wet ice
Categorias relacionadas
Descrição geral
In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body′s response to insulin. Also, its anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack.
The MILLIPLEX® Rat Adipocyte Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
The MILLIPLEX® Rat Adipocyte Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
The MILLIPLEX® MAP Rat Adipocyte Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα.
Especificidade
Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Aplicação
- Analytes: Adiponectin, IL-1β, IL-6, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
- Recommended Sample Type: Rat cell/tissue culture supernatant or lysates.
- Recommended Sample Dilution: 25 μL per well of cell/Tissue culture sample. Samples may require a dilution with an appropriate control medium.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Endocrine; Obesity; Metabolic Disease
Características e benefícios
Design your multiplex kit by choosing available analytes within this panel.
Embalagem
Everything you need in a single kit.
Componentes
Rat Adipokine Standard, 1 vial, lyophilized
Rat Adipokine Quality Control 1, 1 vial, lyophilized
Rat Adipokine Quality Control 2, 1 vial, lyophilized
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Rat Adipocyte Detection Antibodies, 1 bottle, 5.5 mL
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
Rat Adipokine Quality Control 1, 1 vial, lyophilized
Rat Adipokine Quality Control 2, 1 vial, lyophilized
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Rat Adipocyte Detection Antibodies, 1 bottle, 5.5 mL
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
Armazenamento e estabilidade
Recommended storage for kit components is 2 - 8°C.
Outras notas
Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.
Informações legais
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Código de classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Monika Kubacka et al.
Pharmaceuticals (Basel, Switzerland), 14(5) (2021-06-03)
Quinazoline α1-adrenoceptors antagonists have been shown to exert moderately favorable effects on the metabolic profile in hypertensive patients. However, based on AntiHypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial (ALLHAT) results, they are no longer recommended as a first
Hamidah Abu Bakar et al.
Cardiovascular research, 113(8), 962-972 (2017-04-04)
Electrical field stimulation (EFS) elicits robust sensory neurogenic relaxation responses in the rat isolated mesenteric arterial bed but these responses are absent or difficult to demonstrate in isolated arteries. We believe that this mismatch is due to the absence of
Irene Bretón Lesmes et al.
Nutricion hospitalaria, 38(Spec No1), 8-14 (2021-02-03)
Nutritional assessment and treatment, necessary for the prevention and treatment of disease-related malnutrition, should be carried out by a multidisciplinary team where each member has well-defined skills and functions, and mechanisms are established to allow adequate coordination, both in the
Salvador Peiró Moreno et al.
Nutricion hospitalaria, 37(6), 1246-1280 (2020-10-16)
Background: the legislation currently regulating the inclusion of Food for Special Medical Purposes (FSMP) among the benefits of the National Health System (SNS) was made approximately 15 years ago. The evolution of the technical characteristics of the FSMP justifies an
Conteúdo relacionado
Multiplex immunoassays save time and sample volume in metabolic syndrome research, providing comprehensive insights into related conditions.
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica