MABT902

Anti-Connexin 43 Antibody, clone P2C4

clone P2C4, from mouse

• Sinônimo(s): Gap junction alpha-1 protein, CX43, Gap junction 43 kDa heart protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: P2C4, monoclonal
• reatividade de espécies: human, rat, mouse
• reatividade da espécie (prevista por homologia): canine (based on 100% sequence homology), hamster (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), rabbit (based on 100% sequence homology), guinea pig (based on 100% sequence homology)
• técnica(s): electron microscopy: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_036699
• nº de adesão UniProt: P08050
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: bovine ... Gja1(281193) ; dog ... Gja1(403418) ; guinea pig ... Gja1(100379273) ; human ... GJA1(2697) ; mouse ... Gja1(14609) ; rabbit ... Gja1(100008935) ; rat ... Gja1(24392) ; rhesus monkey ... Gja1(714344)

Descrição

Descrição geral

Gap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.

Especificidade

Clone P2C4 detects Connexin 43 in human heart. It targets an epitope within 23 amino acids from the C-terminal region. It primarily binds to the non-phosphorylated isoform around Ser364/365.

Imunogênio

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of rat connexin 43.

Aplicação

Anti-Connexin 43 Antibody, clone P2C4, Cat. No. MABT902, is a mouse monoclonal antibody that targets Connexin-43 and is tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Connexin 43 in rat heart tissue.

Immunoprecipitation Analysis: A representative lot detected Connexin 43 in Immunoprecipitation applications (Solan, J.L., et. al. (2003). J Cell Sci. 116(Pt 11):2203-11).

Electron Microscopy Analysis: A representative lot detected Connexin 43 in Electron Microscopy applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

Qualidade

Evaluated by Immunohistochemistry in human heart tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Connexin 43 in human heart tissue.

Descrição-alvo

43.03 kDa calculated.

forma física

Format: Purified

Outras notas

Concentration: Please refer to lot specific datasheet.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1