MABT533

Anti-PECAM-1 Antibody, clone 4A8.1

clone 4A8.1, from mouse

• Sinônimo(s): Platelet endothelial cell adhesion molecule, CD31, EndoCAM, GPIIA′, PECA1, PECAM-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 4A8.1, monoclonal
• reatividade de espécies: human
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_000433
• nº de adesão UniProt: P16284
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PECAM1(5175)

Descrição

Descrição geral

Platelet endothelial cell adhesion molecule (UniProt P16284; also known as CD31, EndoCAM, GPIIA′, PECA1, PECAM-1) is encoded by the PECAM1 gene (Gene ID 5175) in human. PECAM-1 (CD31) is a 130 kDa cell adhesion molecule that is expressed at the junctions of all continuous endothelia, as well as most bone marrow-derived hematopoietic cells, including platelets, monocytes, granulocytes and some lymphocytes. The human PECAM1 and murine Pecam1 genes both produce multiple spliced isoforms with varying lengths in cytoplasmic domain, with the isoform Long being the major form expressed in human tissues and endothelial cells (ECs), and isoform delta 14-15 being the predominantly expressed murine form. PECAM-1 plays an import role in maintaining vascular integrity and function by mediating ECs interactions with pericytes and smooth muscle cells (SMCs). PECAM-1 is initially produced with a signal peptide (human a.a. 1-27, murine a.a. 1-17) sequence, the removal of which yields the mature protein with a large extracellular region (human a.a. 28-601, mouse a.a. 18-590) composed of six C2-type Ig-like domains, followed by a transmembrane seqment (human a.a. 602-620, mouse a.a. 591-609) and a cytoplasmic tail (human a.a. 621-738, mouse a.a. 610-727) that plays an essential role in PECAM-1-mediated cellular signaling.

Especificidade

Clone 4A8.1 was raised against the cytoplasmic domain of PECAM-1 (CD31) isoform Long. Reactivity toward spliced isoforms Delta12, Delta13, Delta14, Delta14-15, and Delta15 with various cytomplasmic domain deletions is possible, but has not not been confirmed.

Imunogênio

Epitope: cytoplasmic domain

GST-tagged recombinant human PECAM-1 cytoplasmic domain.

Aplicação

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PECAM-1 in human cerebellum, lung, and pancreas tissue sections.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PECAM-1 in 10 µg of Jurkat cell lysate.

Research Category
Cell Structure

This mouse monoclonal Anti-PECAM-1 Antibody, clone 4A8.1, Cat. No. MABT533, is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of PECAM-1 (CD31).

Qualidade

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PECAM-1 in 10 µg of THP-1 human acute monocytic leukemia cell lysate.

Descrição-alvo

~130 kDa observed. Target band size appears larger than the calculated molecular weights of 79.58/77.52/77.25/77.46/76.30/78.42 kDa (mature isoform Long/Delta12/Delta13/Delta14/Delta14-15/Delta15) and 82.54/80.48/80.21/80.42/79.26/81.38 kDa (isoform Long/Delta12/Delta13/Delta14/Delta14-15/Delta15 pro-form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Ligação

Replaces: 04-1074

forma física

Format: Purified

Protein G purified.

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1