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MABT186

Sigma-Aldrich

Anti-Exo70 Antibody, clone 70X13F3

clone 70X13F3, from mouse

Sinônimo(s):

Exocyst complex component 7, Exocyst complex component Exo70, rExo70

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

70X13F3, monoclonal

reatividade de espécies

human, rat

técnica(s)

immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG2bκ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... EXOC7(23265)

Descrição geral

The exocyst complex component 70 (Exo70) is a component of the exocyst complex, an octameric structure that is also comprised of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84. The exocyst complex facilitates the fusion of secretory vesicles with the plasma membrane and the release of vesicular cargo, which may include neurotransmitters, hormones, and membrane proteins. Previous studies have indicated that Exo70 plays an important role in actin assembly and cell motility.

Imunogênio

GST-tagged recombinant protein corresponding to rat Exo70.

Aplicação

Detect Exo70 using this Anti-Exo70 Antibody, clone 70X13F3 validated for use in Western Blotting, ICC.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected Exo70 in 10 µg of human brain tissue lysate.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in Exo70 cells. (Hsu, S.C., et al. (2001). The Journal of Neuroscience. 21(11):3839–3848.)
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hsu, S.C., et al. (2011). The Journal of Neuroscience. 31(10):3522–3535.)

Qualidade

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Exo70 in 10 µg of rat brain tissue lysate.

Descrição-alvo

~75 kDa observed

forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Rat brain tissue lysate

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Cristina Cacho-Navas et al.
Cellular and molecular life sciences : CMLS, 79(1), 61-61 (2022-01-10)
Apical localization of Intercellular Adhesion Receptor (ICAM)-1 regulates the adhesion and guidance of leukocytes across polarized epithelial barriers. Here, we investigate the molecular mechanisms that determine ICAM-1 localization into apical membrane domains of polarized hepatic epithelial cells, and their effect
Cristina Cacho-Navas et al.
eLife, 12 (2024-04-10)
Epithelial intercellular adhesion molecule (ICAM)-1 is apically polarized, interacts with, and guides leukocytes across epithelial barriers. Polarized hepatic epithelia organize their apical membrane domain into bile canaliculi and ducts, which are not accessible to circulating immune cells but that nevertheless

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