MABN14

Anti-phospho TDP-43 (Ser409/Ser410) Antibody, clone 1D3

clone 1D3, from rat

• Sinônimo(s): TAR DNA-binding protein 43, TDP-43, TDP43, TAR DNA binding protein-43, Phosphorylated TDP
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rat
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 1D3, monoclonal
• reatividade de espécies: human
• técnica(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_031401
• nº de adesão UniProt: Q13148
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pSer409/pSer410)
• Informações sobre genes: human ... TARDBP(23435)

Descrição

Descrição geral

TAR DNA-binding protein 43 (TDP-43) belongs to the hnRNP protein family and plays an important role in transcription, pre-mRNA splicing, mRNA stability and mRNA transport. It is involved in splicing of the apolipoprotein A-II and cystic fibrosis transmembrane gene. This protein is highly expressed in the pancreas, placenta, lung, genital tract and spleen. Mutations in TDP-43 have been associated with amyotrophic lateral sclerosis, frontotemporal dementia, Parkinson′s disease and Alzheimer′s disease.

Especificidade

This antibody recognizes TDP-43 when phosphorylated at Ser409/Ser410.

Imunogênio

Epitope: Ser409/Ser410

Ovalbumin-conjugated linear peptide corresponding to human TDP-43 phosphorylated at Ser409/Ser410.

Aplicação

Anti-phospho TDP-43 (Ser409/Ser410) Antibody, clone 1D3 is an antibody against phospho TDP-43 (Ser409/Ser410) for use in WB, ELISA, IH.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in hippocampus from FTLD-U brain tissue. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)

ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Qualidade

Evaluated by Western Blot in human placenta cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected phosphorylated TDP-43 on 10 µg of human placenta cell lysate.

Descrição-alvo

50/25 kDa Observed
1) The 25-kDa C-terminal fragment is more prone to phosphorylation at Ser409/Ser410 than full-length TDP-43. (Zhang, Y.J., et al. (2009). PNAS. 106:7607-7612.)
2) 50-52 kDa observed MW . (Buratti, E., et al. (2001). The EMBO Journal 20:1774-1784.)

forma física

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Human placenta cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10-13 - German Storage Class 10 to 13