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MABE1017

Sigma-Aldrich

Anti-OASIS/CREB3L1 Antibody, clone 44C7

clone 44C7, from mouse

Sinônimo(s):

Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS, Processed cyclic AMP-responsive element-binding protein 3-like protein 1, OASIS

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

44C7, monoclonal

reatividade de espécies

mouse

técnica(s)

western blot: suitable

Isotipo

IgG1κ

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

mouse ... Creb3L1(26427)

Categorias relacionadas

Descrição geral

Cyclic AMP-responsive element-binding protein 3-like protein 1 (UniProt Q9Z125; also known as BBF-2 homolog, cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance) is encoded by the Creb3l1 (also known as Oasis) gene (Gene ID 26427) in murine species. Creb3l1 belongs to the the old astrocyte specifically induced substance (OASIS) family of transcription factors that function as ER stress transducers. OASIS members possess both a transcription-activation domain and a basic leucine zipper (bZIP) domain, as well as a transmembrane domain that allows them to associate with the ER. OASIS family members, except Luman (Creb3), exhibit cell- and tissue-specific expression patterns, with Oasis (Creb3l1) being preferentially expressed in osteoblasts and astrocytes, AIbZIP (Creb3l4, Creb4, Tisp40) in testis and prostate, Bbf2h7 (Creb3l2) in chondrocytes, and Crebh (Creb3l3) in hepatocytes. OASIS family members are activated in response to ER stress via a distinct mechanism than the ER unfold protein response (UPR) transducer ATF6. Oasis (Creb3l1) and Bbf2h7 (Creb3l2) are constantly ubiquitinated by the ER-resident E3 ubiquitin ligase HMG-CoA reductase degradation 1 (Hrd1) and degraded by proteasome under normal conditions. ER stress stabilizes Oasis and Bbf2h7 by inducing a dissociation of these transcription factors from Hrd1.

Especificidade

Expected to react with both spliced isoforms as well as the N-terminal fragment of cleaved Oasis.

Imunogênio

Epitope: N-terminal half
Flag-tagged recombinant protein corresponding to the N-terminal half of mouse OASIS/CREB3L1.

Aplicação

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-OASIS/CREB3L1 Antibody, clone 44C7 is validated for use in Western Blotting for the detection of OASIS/CREB3L1.
Western Blotting Analysis: A representative lot detected full-length OASIS in lysates from untreated MC3T3-E1 murine osteoblastic cells and cleaved N-terminal OASIS fragment in lysates from MC3T3-E1 cells subjected to ER stresser thapsigargin or brefeldin A treatment (Murakami, T., et al. (2009). Nat. Cell Biol. 11(10):1205-1211).
Western Blotting Analysis: A representative lot detected a higher OASIS expression level in Hrd1-/- than in wild-type MEFs. A more elevated OASIS level was also detected in osteoblasts differentiated from Hrd1-/- than from wild-type MEFs following bone morphogenetic protein 2 (BMP2) treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).

Qualidade

Evaluated by Western Blotting in MC3T3-E1 mouse osteoblastic cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected OASIS/CREB3L1 in 10 µg of MC3T3-E1 mouse osteoblastic cell lysate.

Descrição-alvo

~62 and 52 kDa observed

forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Yoshiaki Miyake et al.
Communications biology, 5(1), 734-734 (2022-07-23)
Podocyte injury is involved in the onset and progression of various kidney diseases. We previously demonstrated that the transcription factor, old astrocyte specifically induced substance (OASIS) in myofibroblasts, contributes to kidney fibrosis, as a novel role of OASIS in the
Daisuke Ariyasu et al.
Endocrinology, 160(11), 2673-2691 (2019-08-23)
Isolated growth hormone deficiency type II (IGHD2) is mainly caused by heterozygous splice-site mutations in intron 3 of the GH1 gene. A dominant-negative effect of the mutant GH lacking exon 3 on wild-type GH secretion has been proposed; however, the
Ayaha Yamamoto et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(2), e21158-e21158 (2020-11-06)
Prevention of kidney fibrosis is an essential requisite for effective therapy in preventing chronic kidney disease (CKD). Here, we identify Old astrocyte specifically induced substance (OASIS)/cAMP responsive element-binding protein 3-like 1 (CREB3l1), a CREB/ATF family transcription factor, as a candidate

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