MABC91
Anti-Atg18 (WIPI-2) Antibody, clone 2A2
clone 2A2, from mouse
Sinônimo(s):
WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
2A2, monoclonal
reatividade de espécies
human
técnica(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotipo
IgG1κ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... WIPI2(26100)
Descrição geral
Atg18, also known as WIPI-2, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.
Especificidade
This antibody recognizes Atg18 at the C-terminus.
Imunogênio
Epitope: C-terminus
Linear peptide corresponding to the C-terminus of human Atg18.
Aplicação
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Atg18 in human colorectal adenocarcinoma tissue.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Atg18 (WIPI-2) Antibody, clone 2A2 is validated for use in WB, IH(P) for the detection of Atg18 (WIPI-2).
Qualidade
Evaluated by Western Blot in human testis tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg18 in 10 µg of human testis tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg18 in 10 µg of human testis tissue lysate.
Descrição-alvo
~49 kDa observed.
6 isoforms exist with molecular weights ranging from 42 kDa to 48 kDa
6 isoforms exist with molecular weights ranging from 42 kDa to 48 kDa
forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Nota de análise
Control
Human testis tissue lysate
Human testis tissue lysate
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Shusaku Shibutani et al.
The Journal of biological chemistry, 292(44), 18052-18061 (2017-08-16)
The mechanistic target of rapamycin complex 1 (mTORC1) is a master regulator of protein synthesis and potential target for modifying cellular metabolism in various conditions, including cancer and aging. mTORC1 activity is tightly regulated by the availability of extracellular amino
Jie Li et al.
Cellular and molecular gastroenterology and hepatology, 17(6), 1007-1024 (2024-02-10)
In the classic form of α1-antitrypsin deficiency (ATD), the misfolded α1-antitrypsin Z (ATZ) variant accumulates in the endoplasmic reticulum (ER) of liver cells. A gain-of-function proteotoxic mechanism is responsible for chronic liver disease in a subgroup of homozygotes. Proteostatic response
Takahiro Shimizu et al.
Human molecular genetics, 32(16), 2623-2637 (2023-06-26)
β-propellers that bind polyphosphoinositides (PROPPINs) are an autophagy-related protein family conserved throughout eukaryotes. The PROPPIN family includes Atg18, Atg21 and Hsv2 in yeast and WD-repeat protein interacting with phosphoinositides (WIPI)1-4 in mammals. Mutations in the WIPI genes are associated with
Yasuhiro Yamamoto et al.
Nature communications, 12(1), 3292-3292 (2021-06-04)
Autophagy regulates primary cilia formation, but the underlying mechanism is not fully understood. In this study, we identify NIMA-related kinase 9 (NEK9) as a GABARAPs-interacting protein and find that NEK9 and its LC3-interacting region (LIR) are required for primary cilia
Norito Tamura et al.
FEBS letters, 591(23), 3819-3830 (2017-11-08)
ATG2 is one of the autophagy-related (ATG) proteins essential for autophagosome formation and localizes to isolation membranes and lipid droplets in mammalian cells. Here, we investigated the requirement of regions in ATG2A for its organellar localization and function. The N-terminal
Artigos
Autophagy is a regulated process involved in cell growth, development, and recycling of cytoplasmic components in cells.
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