MABC46
Anti-Atg13 Antibody, clone 2H4.2
clone 2H4.2, from mouse
Sinônimo(s):
Autophagy-related protein 13
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
2H4.2, monoclonal
reatividade de espécies
human
técnica(s)
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG1κ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... ATG13(9776)
Descrição geral
The autophagy-related protein 13 (Atg13) plays an important role in the formation of autophagosomes. Autophagosomes are formed in response to nutrient deprivation and function as the transport vesicles for organelles, proteins, and protein complexes targeted for lysosomes that digest these cargos to produce energy and nutrients. Atg13 is activated by the mTOR pathway and forms a complex with the FIP200 protein. This complex is involved in enhancing the activity of the ULK1 kinase which is required for the formation of autophagosomes. Atg13/FIP200 faciliates the localization of ULK1 to pre-autophagosomes, and subsequently stabilizes ULK1. Autophagy is an important process in development, growth, and cell differentiation, and disruption of this process may contribute to cancer, aging, and neurodegenerative diseases.
Imunogênio
GST-tagged recombinant protein corresponding to human Atg13.
Aplicação
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Atg13 Antibody, clone 2H4.2 is validated for use in Western Blotting, IHC for the detection of Atg13.
Western Blot Analysis: A representative lot detected Atg13 in HeLa cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Atg13 in normal human substancia nigra tissues.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Atg13 in normal human substancia nigra tissues.
Qualidade
Evaluated by Western Blot in campthothecin treated HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Atg13 in 10 µg of campthothecin treated HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Atg13 in 10 µg of campthothecin treated HeLa cell lysate.
Descrição-alvo
~60 kDa observed
forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Nota de análise
Control
Campthothecin treated HeLa cell lysate.
Campthothecin treated HeLa cell lysate.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Yo-Hei Yamamoto et al.
The Journal of cell biology, 219(8) (2020-06-04)
In macroautophagy, membrane structures called autophagosomes engulf substrates and deliver them for lysosomal degradation. Autophagosomes enwrap a variety of targets with diverse sizes, from portions of cytosol to larger organelles. However, the mechanism by which autophagosome size is controlled remains
Correction: ERdj8 governs the size of autophagosomes during the formation process.
Yo-Hei Yamamoto et al.
The Journal of cell biology, 220(9) (2021-08-07)
Naoki Tamura et al.
Molecular and cellular biology, 39(16) (2019-06-05)
Autophagy is considered an adaptive mechanism against hyperosmotic stress. Although the process has been reported to be triggered by the inhibition of mTORC1, the precise downstream mechanisms remain elusive. Here, we demonstrate that hyperosmotic-stress-induced autophagy is different from conventional macroautophagy
Ubiquitination Is a Novel Post-Translational Modification of VMP1 in Autophagy of Human Tumor Cells.
Felipe J Renna et al.
International journal of molecular sciences, 24(16) (2023-08-26)
Autophagy is a tightly regulated catabolic process involved in the degradation and recycling of proteins and organelles. Ubiquitination plays an important role in the regulation of autophagy. Vacuole Membrane Protein 1 (VMP1) is an essential autophagy protein. The expression of
Eleftherios Karanasios et al.
Nature communications, 7, 12420-12420 (2016-08-12)
Autophagosome formation requires sequential translocation of autophagy-specific proteins to membranes enriched in PI3P and connected to the ER. Preceding this, the earliest autophagy-specific structure forming de novo is a small punctum of the ULK1 complex. The provenance of this structure
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