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MAB3868

Sigma-Aldrich

Anti-DNA Antibody, single stranded

clone TNT-3, Chemicon®, from mouse

Sinônimo(s):

ssDNA

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

TNT-3, monoclonal

reatividade da espécie (prevista por homologia)

all

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)

Isotipo

IgG1, kappa

Condições de expedição

dry ice

modificação pós-traducional do alvo

unmodified

Categorias relacionadas

Especificidade

Reacts with single stranded DNA (ssDNA). The antibody is useful for staining nucleated cells. The antibody can also be used to identify necrotic regions of tumors and damaged tissues. By immunohistochemistry, stains heterochromatic regions of cell nucleus.

Imunogênio

Human Raji cell nuclei

Aplicação

Detect DNA using this Anti-DNA Antibody, single stranded validated for use in ELISA, FC, IC, IH(P).
Immunocytochemistry on paraformaldehyde fixed cells

Immunohistochemistry on paraformaldehyde or B5 fixed tissue sections.

Flow cytometry: 5-10 mg/mL using 2% paraformaldehyde fixed cells.

ELISA

Optimal working dilutions must be determined by end user.

STAINING PATTERN:

Stains heterochromatic regions of cell nucleus.
Research Category
Epitope Tags & General Use
Research Sub Category
Organelle & Cell Markers

forma física

Format: Purified
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Francesco Nannini et al.
mAbs, 13(1), 1864084-1864084 (2021-01-01)
Phage display technology in combination with next-generation sequencing (NGS) currently is a state-of-the-art method for the enrichment and isolation of monoclonal antibodies from diverse libraries. However, the current NGS methods employed for sequencing phage display libraries are limited by the
New histone supply regulates replication fork speed and PCNA unloading.
Mejlvang, J; Feng, Y; Alabert, C; Neelsen, KJ; Jasencakova, Z; Zhao, X; Lees, M; Sandelin et al.
The Journal of cell biology null
LiJun Wang et al.
Cellular reprogramming, 23(2), 99-107 (2021-04-17)
Zygotic epigenetic reprogramming is the major initial event in embryo development to acquire a totipotent potential. However, the patterns of epigenetic modifications in bovine zygote were not well clarified, especially in the first cell cycle of bovine somatic cell nuclear
Christiane B de Araujo et al.
The Journal of eukaryotic microbiology, 66(3), 514-518 (2018-08-05)
Here, we investigated the features of replication in Trypanosoma cruzi epimastigotes based on fork speed progression, which is influenced by distinct features such as DNA polymerase rate, susceptibility to DNA damage and repair, secondary structures, transcription and chromatin state. Although
Yunpeng Feng et al.
The EMBO journal, 35(2), 176-192 (2015-12-02)
During DNA replication, thousands of replication origins are activated across the genome. Chromatin architecture contributes to origin specification and usage, yet it remains unclear which chromatin features impact on DNA replication. Here, we perform a RNAi screen for chromatin regulators

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