MAB19562X

Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor^™ 488 conjugated

clone D4B5, Chemicon^®, from mouse

• Sinônimo(s): Anti-B2T, Anti-CSF, Anti-EBR2, Anti-EBR2A, Anti-LAMB2T, Anti-LAMNB2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• conjugado: ALEXA FLUOR^™ 488
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: D4B5, monoclonal
• reatividade de espécies: human
• fabricante/nome comercial: Chemicon^®
• técnica(s): flow cytometry: suitable; immunocytochemistry: suitable
• Isotipo: IgG1
• nº de adesão NCBI: NM_005562.2; NM_018891.2
• nº de adesão UniProt: Q13753
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... LAMC2(3918)

Descrição

Descrição geral

Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through binding with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Compared to fibronectin, collagen, or vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Recently, Laminin-5 is attracting attention as a substrate for carcinoma cells that may stimulate migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, et al., 2002, Lohi, et al., 2001).

Especificidade

Monoclonal antibody MAB19562 was developed against amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein. The antibody recognizes the 150 kDa (precursor form) and 105 kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.

Imunogênio

Amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein.

Epitope: amino acids 382-608

Aplicação

Immunocytochemistry: 2 μg/mL
Flow cytometry
Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor 488 conjugated is validated for use in FC, IC for the detection of Laminin-5.

forma física

Purified immunoglobulin conjugated to Alexa Fluor^™ 488. Liquid in PBS containing 1% BSA, 0.05% Tween-20 and 0.05% sodium azide.

Armazenamento e estabilidade

Maintain at 2°-8°C for up to 12 months from date of receipt.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

ALEXA FLUOR is a trademark of Life Technologies

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Alexa Fluor^™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable