HTS130RTA
Ready-to-Assay LPA3 Lysophospholipid Receptor Frozen Cells
Human
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
41106514
eCl@ss:
32011203
NACRES:
NA.84
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
Nome do produto
Ready-to-Assay LPA3 Lysophospholipid Receptor Frozen Cells,
fonte biológica
human
fabricante/nome comercial
Ready-to-Assay
técnica(s)
calcium flux assay: suitable
nº de adesão NCBI
método de detecção
fluorometric
Descrição geral
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Lysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA3 is expressed primarily in testes, prostate, heart and pancreas. Mice lacking LPA3 have low expression of uterine COX-2 and display decreased uterine implantation and embryo spacing (Ye et al., 2005). Millipore′s cloned human LPA3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant LPA3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at LPA3.
Lysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA3 is expressed primarily in testes, prostate, heart and pancreas. Mice lacking LPA3 have low expression of uterine COX-2 and display decreased uterine implantation and embryo spacing (Ye et al., 2005). Millipore′s cloned human LPA3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant LPA3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at LPA3.
Descrição de linhagem celular
- GPCR Cell Lines
- Host cells: Chem-1
Aplicação
Human LPA3 / EDG7 GPCR frozen cells for Calcium Flux FLIPR Assays.
Ações bioquímicas/fisiológicas
- GPCR Class: A
- Protein Target: LPA3/ EDG7
- Target Sub-Family: Lysophospholipid
Componentes
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
Exoneração de responsabilidade
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Active Filters
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica
