GR32L
Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2)
lyophilized, clone BuGR2, Calbiochem®
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
BuGR2, monoclonal
Formulário
lyophilized
contém
≤0.1% sodium azide as preservative
reatividade de espécies
guinea pig, rabbit, rat, mouse, sheep
não deve reagir com
amphibian, primate, avian
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
Isotipo
IgG2a
Condições de expedição
ambient
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
mouse ... Nr3C1(14815)
Descrição geral
Anti-Glucocorticoid Receptor (Ab-2), mouse monoclonal, clone BuGR2, recognizes ~94-97 kDa glucocorticoid receptor in mouse liver. It is validated for WB, ICC, IP & IHC (Frozen & paraffin sections).
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-AgX-653 myeloma cells (see application references). Recognizes the ~94-97 kDa glucocorticoid receptor.
Recognizes the ~94-97 kDa glucocorticoid receptor in mouse liver tissue.
Imunogênio
Rat
partially purified, rat glucocorticoid receptor
Aplicação
Frozen Sections (5 g/ml)
Immunoblotting (5 g/ml)
Immunocytochemistry (5 g/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 g/ml)
Advertência
Toxicity: Standard Handling (A)
forma física
Lyophilized from PBS, pH 7.2.
Reconstituição
Resuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Nota de análise
Positive Control
mouse liver extract
mouse liver extract
Outras notas
Does not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA94, 1521.
Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev.18 306.
Schumacher, M., et al. 1996. Dev. Neurosci.18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci.18, 36.
Weigel, N.L. 1996. Biochem J.319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci.743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev.18 306.
Schumacher, M., et al. 1996. Dev. Neurosci.18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci.18, 36.
Weigel, N.L. 1996. Biochem J.319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci.743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Mohammad M Khan et al.
Neuroendocrinology, 84(5), 317-329 (2006-12-05)
MNAR/PELP1 (see text) is a newly identified scaffold protein/coactivator initially thought to modulate nongenomic and genomic actions of the estrogen receptor; however, it has been recently shown to interact with multiple steroid receptors, including androgen and glucocorticoid receptors. In the
FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells.
Biswajyoti Sahu et al.
Cancer research, 73(5), 1570-1580 (2012-12-28)
The forkhead protein FoxA1 has functions other than a pioneer factor, in that its depletion brings about a significant redistribution in the androgen receptor (AR) and glucocorticoid receptor (GR) cistromes. In this study, we found a novel function for FoxA1
Nina M Patrick et al.
The Journal of steroid biochemistry and molecular biology, 167, 1-13 (2016-09-21)
Small molecule inhibitors of lysine deacetylases (KDACs) are approved for clinical use in treatment of several diseases. Nuclear receptors, such as the glucocorticoid receptor (GR) use lysine acetyltransferases (KATs or HATs) and KDACs to regulate transcription through acetylation and deacetylation
Vineela Kadiyala et al.
The Journal of biological chemistry, 288(40), 28900-28912 (2013-08-16)
Nuclear receptors use lysine acetyltransferases and lysine deacetylases (KDACs) in regulating transcription through histone acetylation. Lysine acetyltransferases interact with steroid receptors upon binding of an agonist and are recruited to target genes. KDACs have been shown to interact with steroid
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