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Merck
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CP28

Sigma-Aldrich

Anti-Fibroblast Antigen Mouse mAb (AS02)

liquid, clone AS02, Calbiochem®

Sinônimo(s):

Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)

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About This Item

Código UNSPSC:
12352203
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

AS02, monoclonal

Formulário

liquid

contém

≤0.1% sodium azide as preservative

reatividade de espécies

human, monkey

não deve reagir com

rat, mouse

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze

Isotipo

IgG1

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... FGF22(27006)

Descrição geral

Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sections).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with X63-Ag.8.653 mouse myeloma cells. Recognizes the ~30-31 kDa fibroblast antigen (CD90/Thy-1) under non-reducing conditions.
Recognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.

Imunogênio

Human
human fibroblasts

Aplicação

Flow Cytometry (see comments)

Frozen Sections (10 µg/ml, acetone fixation)

Immunoblotting (10 µg/ml, non-reducing only)

Immunofluorescence (10 µg/ml)

Immunoprecipitation (2-10 µg/ml)

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

forma física

In 50 mM sodium phosphate buffer, 6% BSA.

Nota de análise

Positive Control
Any fibroblast cell line or skin tissue

Outras notas

Saalbach, A., et al. 1996 J. Invest. Dermatol.106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
This antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.

Informações legais

Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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