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Merck
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Documentos Principais

AP202

Sigma-Aldrich

Goat Anti-Rat light chain Antibody

Chemicon®, from goat

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

goat

conjugado

unconjugated

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

reatividade de espécies

rat

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
western blot: suitable

Isotipo

IgG

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Descrição geral

Light chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain′s mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Especificidade

Minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep.
The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins.

Imunogênio

Epitope: Kappa light chain
Prepared from rat IgG light chain.

Aplicação

Goat anti-Rat light chain Antibody is an antibody against light chain for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Western Blotting

ELISA

Optimal working dilutions must be determined by the end user.

Descrição-alvo

25 kDa

forma física

ImmunoAffinity Purified
Purified by immunoaffinity chromatography. Liquid in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6.

Armazenamento e estabilidade

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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