ABS437

Anti-RSPO1 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): R-spondin1, RSPO, CRISTIN3, Roof plate-specific spondin-1, hRspo1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: rat, human
• técnica(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• nº de adesão UniProt: Q2MKA7
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... RSPO1(284654)

Descrição

Descrição geral

R-spondin-1 (RSPO1), or Roof plate-specific spondin-1 protein, is potent activator of the Wnt/beta-catenin signaling pathway leading to TCF/LEF (T cell-specific transcription factor/lymphoid enhancer factor) gene activation. RSPO1 operates on both the canonical Wnt/beta-catenin and non-canonical Wnt signaling pathways by acting as an inhibitor of ZNRF3 (zinc and ring finger 3) protein, a cell surface transmembrane E3 ubiquitin ligase, which is an important regulator of Wnt signaling. RSPO1 interacts with the extracellular domain of ZNRF3 and induces ZNRF3 membrane clearance. RSPO1 is also part of a family of secreted proteins that play critical roles in vertebrate development, including female sex development, and RSPO1 is required for early development of the gonads regardless of sex. Molecularly, RSPO1’s activities synergize with those of WNT4 and act to stabilize beta catenin which then activates its downstream targets. RSPO1 has been shown to be a potent mitogen for specific cell types such as gastrointestinal epithelium, and it is being examined as a key target for colon cancer therapy and for the treatment of mucositis (inflammation of the oral cavity, which is common among patients receiving chemotherapy or radiation for head and neck cancers). Recently RSPO1 was also shown to be a novel beta-cell line growth factor and a factor that promotes insulin secretion in a glucose independent fashion, thus making it a target for enhancing beta-cell function in patients with type 2 diabetes. Defects in RSPO1 can lead to female to male sex reversal and have been associated with the genetic disease PKKSCC (palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal). EMD Millipore’s Anti-RSPO1 rabbit polyclonal antibody has been tested in western blotting, immunohistochemistry, and immunofluorescence in human and rat samples.

Imunogênio

KLH-conjugated linear peptide corresponding to human RSPO1.

Aplicação

Detect R-spondin-1 using this rabbit polyclonal antibody, Anti-RSPO1 Antibody validated for use in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected RSPO1 in human spleen tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected RSPO1 in human spleen cells.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Qualidade

Evaluated by Western Blotting in rat spleen tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected RSPO1 in 15 µg of rat spleen tissue lysate.

Descrição-alvo

~37 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 3 isoforms at ~22 kDa, ~26 kDa, and ~29 kDa

forma física

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Rat spleen tissue lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable