ABE208

Anti-β-Catenin Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): catenin (cadherin-associated protein), beta 1, 88kDa, catenin (cadherin-associated protein), beta 1 (88kD)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: mouse, human, rat, horse
• reatividade da espécie (prevista por homologia): ox (based on 100% sequence homology), equine (based on 100% sequence homology)
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• nº de adesão NCBI: NP_001895
• nº de adesão UniProt: P35222
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: horse ... Ctnnb1(100055241) ; human ... CTNNB1(1499) ; mouse ... Ctnnb1(539003) , Ctnnb1(12387) ; rat ... Ctnnb1(84353)

Descrição

Descrição geral

β-catenin is the founding member of the β -catenin family. This protein is comprised of 12 ARM repeats and is considered to have dual function depending on its localization. Cytoplasmic β -catenin is a critical component of E-cadherin/catening adhesion complexes and is involved in regulating cadherin-dependent cell-cell interactions.When localized to the nucleus, β -catenin serves a regulatory role by binding with transcription factors from the TCF/LEF family, activating transcription. Expression of β -catenin has been reported in a variety of hair follicle cells such as cells found in the inner and outer portions of the root sheaths, and the basal and peripheral matrix cells. It has also been observed in colon tissue. Defects in expression have been linked to the development of several tumors and cancers such as; colorectal cancer (CRC), medulloblastoma (MDB), ovarian cancer (OC), and pilomatrixoma (PTR).

Especificidade

Antibody recognizes β-Catenin at the N-terminus.

Imunogênio

Epitope: Consensus GSK3 phosphorylation site of human β-Catenin

KLH-conjugated linear peptide corresponding to the consensus GSK3 phosphorylation site of human β-Catenin.

Aplicação

Anti-β-Catenin Antibody is a Rabbit Polyclonal Antibody for detection of β-Catenin also known as catenin (cadherin-associated protein) beta 1 (88kD) & has been validated in WB, ICC & IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated β-Catenin from A431 lysate.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected β-Catenin in NIH/3T3 and A431 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualidade

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected β-Catenin on 10 µg of A431 cell lysate.

Descrição-alvo

~86kDa observed.

Ligação

Replaces: 04-1002

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
A431 cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable